REMEDIUMREMEDIUM1561-59362658-3534Joint-Stock Company Chicot180910.32687/1561-5936-2025-29-3-227-230Original ArticleImprovement of production of standard isohemagglutinizing serum ABO kits from the point of import substitution and reduction of financial costsТураеваРоза Рамильевнаturaeva_roza@bk.ruТураевРамиль Габдельхаковичramil.turaev@tatar.ruХакимоваРамзия Ильгизовнаdls.rck@tatar.ruЗариповаРегина Маратовнаdls.rck@tatar.ruМингазоваЭльмира Нурисламовнаelmira_mingazova@mail.ruЖибуртЕвгений Борисович006Republican Clinical Hospital of the Ministry of Health of the Republic of Tatarstan, Kazan, RussiaRepublican Blood Center of the Ministry of Health of the Republic of Tatarstan, Kazan, RussiaN. A. Semashko National Research Institute of Public Health, Moscow, RussiaRussian Medical Academy of Continuous Professional Education, Moscow, RussiaKazan State Medical University, Kazan, RussiaPirogov National Medical and Surgical Center, Ministry of Health of Russia, Moscow, Russia15122025322723017102025Copyright © 2025,2025The aim of this study was to prepare proposals to improve the production of standard ABO isohemagglutinin serum kits based on an assessment of the phenotypic, temporal, and gender characteristics of the prevalence of high-titer regular antibodies of the ABO system. Materials and methods. In 2021—2023, the Republican Blood Center of the Ministry of Health of the Republic of Tatarstan conducted a study of the titer of natural group antibodies in blood samples of all donors, except for group AB, in order to detect high (1:256 and higher) titers necessary for the production of standard ABO isohemagglutinin serum. A total of 38,795 donors were examined, high-titer regular antibodies of the ABO system were detected in 58 (15.0 ‰O). The phenotypic, temporal, and gender characteristics of the occurrence of high-titer regular antibodies of the ABO system were assessed. Results. The proportion of women among donors was 29.8%. Over the entire observation period, high-titer regular antibodies of the ABO system were found in women by 19.3 ‰O more often than in men (plt;0.001). The frequency of occurrence of high-titer regular antibodies of the ABO system has no gender differences in the O and A phenotypes and is significantly higher in female donors of the B phenotype by 51.0 ‰O (plt;0.001). Anti-B are less common than anti-A by 11.8 ‰O (plt;0.03). Conclusion. The revealed increased frequency of anti-A in female donors of the Republic of Tatarstan allows us to recommend examining no more than 50% of female donors for high-titer regular antibodies and completely abandoning the search for these antibodies in men of group B. Based on the experience of 2021—2023 such a reduction would amount to 8662 out of 38795 studies or a reduction in material and labor costs by 22.3%.ABO blood group system, donors, blood components, immunohematology, antibodies, immune antibodiesсистема группы крови АВО, доноры, компоненты крови, иммуногематология, антитела, иммунные антитела1.Zhiburt E. B., Chemodanov I. G., Averyanov E. G., Kozhemyako O. V. Sustainability of blood services. Bulletin of the National Research Institute of Public Health named after N. A. Semashko. 2017;(7):17—24 (In Russ.).2.Shevchenko Yu. L., Karpov O. E., Zhiburt E. B. Blood transfusion: history and modernity (on the 100th anniversary of blood transfusion in Russia). Bulletin of the Pirogov National Medical and Surgical Center. 2019;14(4):4—11 (In Russ.). DOI: 10.25881/BPNMSC.2020.29.78.0013.Zhiburt E. B., Shestakov E. A., Kuznetsov S. I. Hemolytic transfusion reactions. Bulletin of the Pirogov National Medical and Surgical Center. 2019;14(4):105—111 (In Russ.). DOI: 10.25881/BPNMSC.2020.17.22.0204.Zolotarev P. N., Cherkasov S. N. The market of medical laboratory services in the Russian Federation. Bulletin of the National Research Institute of Public Health named after N. A. Semashko. 2018;(4):14—18 (In Russ.). DOI: 10.25742/NRIPH.2018.04.0115.Zhiburt E. B. Transfusiological dictionary. Moscow: RAEN; 2012 (In Russ.).